Comparative characterization of extracellular enzymes secreted by Phanerochaete chrysosporium during solid-state and submerged fermentation.
Identifieur interne : 000037 ( Main/Exploration ); précédent : 000036; suivant : 000038Comparative characterization of extracellular enzymes secreted by Phanerochaete chrysosporium during solid-state and submerged fermentation.
Auteurs : Jiawen Liu [République populaire de Chine] ; Jinshui Yang [République populaire de Chine] ; Ruonan Wang [République populaire de Chine] ; Liang Liu [République populaire de Chine] ; Yu Zhang [République populaire de Chine] ; Huifang Bao [République populaire de Chine] ; Jin Myong Jang [République populaire de Chine] ; Entao Wang [Mexique] ; Hongli Yuan [République populaire de Chine]Source :
- International journal of biological macromolecules [ 1879-0003 ] ; 2020.
Abstract
Influence of water content on the expression of lignocellulolytic enzymes by Phanerochaete chrysosporium remains unclear. This work compares the enzyme production profiles of P. chrysosporium during solid-state and submerged fermentation. There were 110 and 64 extracellular carbohydrate-active enzymes identified in solid-state and submerged fermentation respectively, among which 57 enzymes were common to both of the secretomes. P. chrysosporium secreted more cellulases (especially lytic polysaccharide monooxygenase) and hemicellulases during solid-state fermentation while the proportion of enzyme containing carbohydrate-binding module was higher for submerged fermentation. Although its activities were weaker, the enzyme cocktail from submerged fermentation was surprisingly more effective in hydrolysis at low substrate loading. This advantage of enzymes from submerged fermentation was mainly attributed to carbohydrate-binding module because more xylanases bound with substrate at the beginning of hydrolysis. These results reveal the influence of fermentation conditions on enzyme produced by P. chrysosporium for the first time and show the importance of carbohydrate-binding module in the hydrolysis process of lignocellulose.
DOI: 10.1016/j.ijbiomac.2020.02.256
PubMed: 32105685
Affiliations:
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Electronic address: rnwang@cau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Liang" sort="Liu, Liang" uniqKey="Liu L" first="Liang" last="Liu">Liang Liu</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Yu" sort="Zhang, Yu" uniqKey="Zhang Y" first="Yu" last="Zhang">Yu Zhang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Bao, Huifang" sort="Bao, Huifang" uniqKey="Bao H" first="Huifang" last="Bao">Huifang Bao</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang</wicri:regionArea><wicri:noRegion>Xinjiang</wicri:noRegion></affiliation></author><author><name sortKey="Jang, Jin Myong" sort="Jang, Jin Myong" uniqKey="Jang J" first="Jin Myong" last="Jang">Jin Myong Jang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Entao" sort="Wang, Entao" uniqKey="Wang E" first="Entao" last="Wang">Entao Wang</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City</wicri:regionArea><wicri:noRegion>Mexico City</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, Hongli" sort="Yuan, Hongli" uniqKey="Yuan H" first="Hongli" last="Yuan">Hongli Yuan</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China. Electronic address: hlyuan@cau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">International journal of biological macromolecules</title><idno type="eISSN">1879-0003</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Influence of water content on the expression of lignocellulolytic enzymes by Phanerochaete chrysosporium remains unclear. This work compares the enzyme production profiles of P. chrysosporium during solid-state and submerged fermentation. There were 110 and 64 extracellular carbohydrate-active enzymes identified in solid-state and submerged fermentation respectively, among which 57 enzymes were common to both of the secretomes. P. chrysosporium secreted more cellulases (especially lytic polysaccharide monooxygenase) and hemicellulases during solid-state fermentation while the proportion of enzyme containing carbohydrate-binding module was higher for submerged fermentation. Although its activities were weaker, the enzyme cocktail from submerged fermentation was surprisingly more effective in hydrolysis at low substrate loading. This advantage of enzymes from submerged fermentation was mainly attributed to carbohydrate-binding module because more xylanases bound with substrate at the beginning of hydrolysis. These results reveal the influence of fermentation conditions on enzyme produced by P. chrysosporium for the first time and show the importance of carbohydrate-binding module in the hydrolysis process of lignocellulose.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32105685</PMID><DateRevised><Year>2020</Year><Month>05</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0003</ISSN><JournalIssue CitedMedium="Internet"><Volume>152</Volume><PubDate><Year>2020</Year><Month>Jun</Month><Day>01</Day></PubDate></JournalIssue><Title>International journal of biological macromolecules</Title><ISOAbbreviation>Int J Biol Macromol</ISOAbbreviation></Journal><ArticleTitle>Comparative characterization of extracellular enzymes secreted by Phanerochaete chrysosporium during solid-state and submerged fermentation.</ArticleTitle><Pagination><MedlinePgn>288-294</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0141-8130(20)30930-2</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ijbiomac.2020.02.256</ELocationID><Abstract><AbstractText>Influence of water content on the expression of lignocellulolytic enzymes by Phanerochaete chrysosporium remains unclear. This work compares the enzyme production profiles of P. chrysosporium during solid-state and submerged fermentation. There were 110 and 64 extracellular carbohydrate-active enzymes identified in solid-state and submerged fermentation respectively, among which 57 enzymes were common to both of the secretomes. P. chrysosporium secreted more cellulases (especially lytic polysaccharide monooxygenase) and hemicellulases during solid-state fermentation while the proportion of enzyme containing carbohydrate-binding module was higher for submerged fermentation. Although its activities were weaker, the enzyme cocktail from submerged fermentation was surprisingly more effective in hydrolysis at low substrate loading. This advantage of enzymes from submerged fermentation was mainly attributed to carbohydrate-binding module because more xylanases bound with substrate at the beginning of hydrolysis. These results reveal the influence of fermentation conditions on enzyme produced by P. chrysosporium for the first time and show the importance of carbohydrate-binding module in the hydrolysis process of lignocellulose.</AbstractText><CopyrightInformation>Copyright © 2020. Published by Elsevier B.V.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Jiawen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Jinshui</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ruonan</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China. Electronic address: rnwang@cau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Liang</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bao</LastName><ForeName>Huifang</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jang</LastName><ForeName>Jin Myong</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Entao</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Hongli</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China. Electronic address: hlyuan@cau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Int J Biol Macromol</MedlineTA><NlmUniqueID>7909578</NlmUniqueID><ISSNLinking>0141-8130</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Carbohydrate-binding module</Keyword><Keyword MajorTopicYN="N">Cellulase</Keyword><Keyword MajorTopicYN="N">Hemicellulase</Keyword><Keyword MajorTopicYN="N">Lignocellulose</Keyword><Keyword MajorTopicYN="N">Phanerochaete chrysosporium</Keyword><Keyword MajorTopicYN="N">Secretome</Keyword></KeywordList><CoiStatement>Declaration of competing interest None.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>02</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>02</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>2</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>2</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32105685</ArticleId><ArticleId IdType="pii">S0141-8130(20)30930-2</ArticleId><ArticleId IdType="doi">10.1016/j.ijbiomac.2020.02.256</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Mexique</li><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Jiawen" sort="Liu, Jiawen" uniqKey="Liu J" first="Jiawen" last="Liu">Jiawen Liu</name></noRegion><name sortKey="Bao, Huifang" sort="Bao, Huifang" uniqKey="Bao H" first="Huifang" last="Bao">Huifang Bao</name><name sortKey="Jang, Jin Myong" sort="Jang, Jin Myong" uniqKey="Jang J" first="Jin Myong" last="Jang">Jin Myong Jang</name><name sortKey="Liu, Liang" sort="Liu, Liang" uniqKey="Liu L" first="Liang" last="Liu">Liang Liu</name><name sortKey="Wang, Ruonan" sort="Wang, Ruonan" uniqKey="Wang R" first="Ruonan" last="Wang">Ruonan Wang</name><name sortKey="Yang, Jinshui" sort="Yang, Jinshui" uniqKey="Yang J" first="Jinshui" last="Yang">Jinshui Yang</name><name sortKey="Yuan, Hongli" sort="Yuan, Hongli" uniqKey="Yuan H" first="Hongli" last="Yuan">Hongli Yuan</name><name sortKey="Zhang, Yu" sort="Zhang, Yu" uniqKey="Zhang Y" first="Yu" last="Zhang">Yu Zhang</name></country><country name="Mexique"><noRegion><name sortKey="Wang, Entao" sort="Wang, Entao" uniqKey="Wang E" first="Entao" last="Wang">Entao Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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